Molecular Cloning, Expression and Characterization of a Squalene Synthase Gene from Grain Amaranth (<i>Amaranthus cruentus</i> L.)
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چکیده
منابع مشابه
Molecular cloning and characterization of the yew gene encoding squalene synthase from Taxus cuspidata.
The enzyme squalene synthase (EC 2.5.1.21) catalyzes a reductive dimerization of two farnesyl diphosphate (FPP) molecules into squalene, a key precursor for the sterol and triterpene biosynthesis. A full-length cDNA encoding squalene synthase (designated as TcSqS) was isolated from Taxus cuspidata, a kind of important medicinal plants producing potent anti-cancer drug, taxol. The full-length cD...
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Background: Taxol is one of the most important anti-cancer drugs, which is obtained from yew trees (<span style="font-variant: normal; ...
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Amino acid sequence information was obtained for the NH2 terminus, and for endogenous peptides generated by trypsin digestion, of a purified, truncated form of rat hepatic squalene synthase (RSS, EC 2.5.1.21) (Shechter, I., Klinger, E., Rucker, M. L., Engstrom, R. G., Spirito, J. A., Islam, M. A., Boettcher, B. R., and Weinstein, D. B. (1992) J. Biol. Chem. 267, 8628-8635). Degenerate primers, ...
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Celastrol is an active triterpenoid compound derived from Tripterygium wilfordii which is well-known as a traditional Chinese medicinal plant. Squalene synthase has a vital role in condensing two molecules of farnesyl diphosphate to form squalene, a key precursor of triterpenoid biosynthesis. In the present study, T. wilfordii squalene synthase (TwSQS) was cloned followed by prokaryotic express...
متن کاملMolecular cloning, characterization, and differential expression of a lanosterol synthase gene from Ganoderma lucidum.
A homology-based PCR method was used to clone a cDNA encoding lanosterol synthase (LS) from Ganoderma lucidum (G. lucidum), which produces triterpenes. The cDNA of the LS (GenBank accession no. GQ169528) was found to contain an open reading frame (ORF) of 2,181 bp encoding a 726 amino acid polypeptide, whereas the LS genomic DNA sequence (GenBank accession no. GQ169529) consists of 2,924 bp. Fu...
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ژورنال
عنوان ژورنال: Japan Agricultural Research Quarterly: JARQ
سال: 2016
ISSN: 0021-3551,2185-8896
DOI: 10.6090/jarq.50.307